The medicinal properties of holy basil have made us to further concentrate on its properties. The methanol and ethanol extracts of the plant Ocimum tenuiflorum was taken using Soxhlet extraction method. The phytochemical profile of both the extracts was observed for certain compounds. The extract was taken in different concentrations and was tested against three bacterial species (Bacillus Subtilis, Staphylococcus aureus and Escherichia coli) and three fungal species (Aspergillus niger, Aspergillus flavus, Candida albicans). The estimation of secondary metabolite, Alkaloid estimation was done and was observed using thin layer chromatograph.